Macroscopic Supramolecular Assembly Strategy to Construct 3D Biocompatible Microenvironments with Site-Selective Cell Adhesion

Three-dimensional (3D) scaffolds with chemical diversity are significant to direct cell adhesion onto targeted surfaces, which provides solutions further control over fates and even tissue formation. However, the site-specific modification of specific biomolecules realize selective has been a challenge current methods when building 3D scaffolds. Conventional immersing as-prepared structures in lead nonselective adsorption; recent printing have address problem switching multiple nozzles containing different biomolecules. The recently developed concept macroscopic supramolecular assembly (MSA) based on idea modular is promising fabricate such advantages flexible design combination diverse modules surface chemistry. Herein we report an MSA method ordered internal for site-selective adhesion. structure prepared via alignment polydimethylsiloxane (PDMS) blocks magnetic pick-and-place operation subsequent interfacial bindings between PDMS host/guest molecular recognition. affinity realized by distributing that modified polylysine molecules opposite chiralities: films poly-l-lysine (PLL) show higher density than those poly-d-lysine (PDL). This principle directed simply spatial distribution chiral proven effective five lines. facile strategy holds promise build complex microenvironment on-demand chemical/biological diversities, meaningful study cell/material interactions